UV-B mediated metabolic rearrangements in poplar revealed by non-targeted metabolomics.
Identifieur interne : 001A74 ( Main/Exploration ); précédent : 001A73; suivant : 001A75UV-B mediated metabolic rearrangements in poplar revealed by non-targeted metabolomics.
Auteurs : Moritz Kaling [Allemagne] ; Basem Kanawati ; Andrea Ghirardo ; Andreas Albert ; Jana Barbro Winkler ; Werner Heller ; Csengele Barta ; Francesco Loreto ; Philippe Schmitt-Kopplin ; Jörg-Peter SchnitzlerSource :
- Plant, cell & environment [ 1365-3040 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- effets des radiations : Photosynthèse, Populus.
- génétique : Populus.
- métabolisme : Hémiterpènes, Phénols, Populus.
- Butadiènes, Microscopie confocale, Métabolome, Métabolomique, Pentanes.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Hemiterpenes, Phenols.
- chemical : Butadienes, Pentanes.
- genetics : Populus.
- metabolism : Populus.
- radiation effects : Photosynthesis, Populus.
- Metabolome, Metabolomics, Microscopy, Confocal.
Abstract
Plants have to cope with various abiotic stresses including UV-B radiation (280-315 nm). UV-B radiation is perceived by a photoreceptor, triggers morphological responses and primes plant defence mechanisms such as antioxidant levels, photoreapir or accumulation of UV-B screening pigments. As poplar is an important model system for trees, we elucidated the influence of UV-B on overall metabolite patterns in poplar leaves grown under high UV-B radiation. Combining non-targeted metabolomics with gas exchange analysis and confocal microscopy, we aimed understanding how UV-B radiation triggers metabolome-wide changes, affects isoprene emission, photosynthetic performance, epidermal light attenuation and finally how isoprene-free poplars adjust their metabolome under UV-B radiation. Exposure to UV-B radiation caused a comprehensive rearrangement of the leaf metabolome. Several hundreds of metabolites were up- and down-regulated over various pathways. Our analysis, revealed the up-regulation of flavonoids, anthocyanins and polyphenols and the down-regulation of phenolic precursors in the first 36 h of UV-B treatment. We also observed a down-regulation of steroids after 12 h. The accumulation of phenolic compounds leads to a reduced light transmission in UV-B-exposed plants. However, the accumulation of phenolic compounds was reduced in non-isoprene-emitting plants suggesting a metabolic- or signalling-based interaction between isoprenoid and phenolic pathways.
DOI: 10.1111/pce.12348
PubMed: 24738572
Affiliations:
Links toward previous steps (curation, corpus...)
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Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, D-85764, Neuherberg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, D-85764, Neuherberg, Germany; Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, D-85764, Neuherberg</wicri:regionArea><wicri:noRegion>85764, Neuherberg</wicri:noRegion><wicri:noRegion>85764, Neuherberg</wicri:noRegion><wicri:noRegion>Neuherberg</wicri:noRegion></affiliation></author><author><name sortKey="Kanawati, Basem" sort="Kanawati, Basem" uniqKey="Kanawati B" first="Basem" last="Kanawati">Basem Kanawati</name></author><author><name sortKey="Ghirardo, Andrea" sort="Ghirardo, Andrea" uniqKey="Ghirardo A" first="Andrea" last="Ghirardo">Andrea Ghirardo</name></author><author><name sortKey="Albert, Andreas" sort="Albert, Andreas" uniqKey="Albert A" first="Andreas" last="Albert">Andreas Albert</name></author><author><name sortKey="Winkler, Jana Barbro" sort="Winkler, Jana Barbro" uniqKey="Winkler J" first="Jana Barbro" last="Winkler">Jana Barbro Winkler</name></author><author><name sortKey="Heller, Werner" sort="Heller, Werner" uniqKey="Heller W" first="Werner" last="Heller">Werner Heller</name></author><author><name sortKey="Barta, Csengele" sort="Barta, Csengele" uniqKey="Barta C" first="Csengele" last="Barta">Csengele Barta</name></author><author><name sortKey="Loreto, Francesco" sort="Loreto, Francesco" uniqKey="Loreto F" first="Francesco" last="Loreto">Francesco Loreto</name></author><author><name sortKey="Schmitt Kopplin, Philippe" sort="Schmitt Kopplin, Philippe" uniqKey="Schmitt Kopplin P" first="Philippe" last="Schmitt-Kopplin">Philippe Schmitt-Kopplin</name></author><author><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Butadienes (MeSH)</term><term>Hemiterpenes (metabolism)</term><term>Metabolome (MeSH)</term><term>Metabolomics (MeSH)</term><term>Microscopy, Confocal (MeSH)</term><term>Pentanes (MeSH)</term><term>Phenols (metabolism)</term><term>Photosynthesis (radiation effects)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (radiation effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Butadiènes (MeSH)</term><term>Hémiterpènes (métabolisme)</term><term>Microscopie confocale (MeSH)</term><term>Métabolome (MeSH)</term><term>Métabolomique (MeSH)</term><term>Pentanes (MeSH)</term><term>Photosynthèse (effets des radiations)</term><term>Phénols (métabolisme)</term><term>Populus (effets des radiations)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hemiterpenes</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Butadienes</term><term>Pentanes</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Photosynthèse</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Hémiterpènes</term><term>Phénols</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Photosynthesis</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Metabolome</term><term>Metabolomics</term><term>Microscopy, Confocal</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Butadiènes</term><term>Microscopie confocale</term><term>Métabolome</term><term>Métabolomique</term><term>Pentanes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plants have to cope with various abiotic stresses including UV-B radiation (280-315 nm). UV-B radiation is perceived by a photoreceptor, triggers morphological responses and primes plant defence mechanisms such as antioxidant levels, photoreapir or accumulation of UV-B screening pigments. As poplar is an important model system for trees, we elucidated the influence of UV-B on overall metabolite patterns in poplar leaves grown under high UV-B radiation. Combining non-targeted metabolomics with gas exchange analysis and confocal microscopy, we aimed understanding how UV-B radiation triggers metabolome-wide changes, affects isoprene emission, photosynthetic performance, epidermal light attenuation and finally how isoprene-free poplars adjust their metabolome under UV-B radiation. Exposure to UV-B radiation caused a comprehensive rearrangement of the leaf metabolome. Several hundreds of metabolites were up- and down-regulated over various pathways. Our analysis, revealed the up-regulation of flavonoids, anthocyanins and polyphenols and the down-regulation of phenolic precursors in the first 36 h of UV-B treatment. We also observed a down-regulation of steroids after 12 h. The accumulation of phenolic compounds leads to a reduced light transmission in UV-B-exposed plants. However, the accumulation of phenolic compounds was reduced in non-isoprene-emitting plants suggesting a metabolic- or signalling-based interaction between isoprenoid and phenolic pathways. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24738572</PMID><DateCompleted><Year>2016</Year><Month>01</Month><Day>25</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>5</Issue><PubDate><Year>2015</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>UV-B mediated metabolic rearrangements in poplar revealed by non-targeted metabolomics.</ArticleTitle><Pagination><MedlinePgn>892-904</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pce.12348</ELocationID><Abstract><AbstractText>Plants have to cope with various abiotic stresses including UV-B radiation (280-315 nm). UV-B radiation is perceived by a photoreceptor, triggers morphological responses and primes plant defence mechanisms such as antioxidant levels, photoreapir or accumulation of UV-B screening pigments. As poplar is an important model system for trees, we elucidated the influence of UV-B on overall metabolite patterns in poplar leaves grown under high UV-B radiation. Combining non-targeted metabolomics with gas exchange analysis and confocal microscopy, we aimed understanding how UV-B radiation triggers metabolome-wide changes, affects isoprene emission, photosynthetic performance, epidermal light attenuation and finally how isoprene-free poplars adjust their metabolome under UV-B radiation. Exposure to UV-B radiation caused a comprehensive rearrangement of the leaf metabolome. Several hundreds of metabolites were up- and down-regulated over various pathways. Our analysis, revealed the up-regulation of flavonoids, anthocyanins and polyphenols and the down-regulation of phenolic precursors in the first 36 h of UV-B treatment. We also observed a down-regulation of steroids after 12 h. The accumulation of phenolic compounds leads to a reduced light transmission in UV-B-exposed plants. However, the accumulation of phenolic compounds was reduced in non-isoprene-emitting plants suggesting a metabolic- or signalling-based interaction between isoprenoid and phenolic pathways. </AbstractText><CopyrightInformation>© 2014 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kaling</LastName><ForeName>Moritz</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Research Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, D-85764, Neuherberg, Germany; Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, D-85764, Neuherberg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kanawati</LastName><ForeName>Basem</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Ghirardo</LastName><ForeName>Andrea</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Albert</LastName><ForeName>Andreas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Winkler</LastName><ForeName>Jana Barbro</ForeName><Initials>JB</Initials></Author><Author ValidYN="Y"><LastName>Heller</LastName><ForeName>Werner</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Barta</LastName><ForeName>Csengele</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Loreto</LastName><ForeName>Francesco</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Schmitt-Kopplin</LastName><ForeName>Philippe</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Schnitzler</LastName><ForeName>Jörg-Peter</ForeName><Initials>JP</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002070">Butadienes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045782">Hemiterpenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010420">Pentanes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0A62964IBU</RegistryNumber><NameOfSubstance UI="C005059">isoprene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002070" MajorTopicYN="N">Butadienes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045782" MajorTopicYN="N">Hemiterpenes</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055442" MajorTopicYN="N">Metabolome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055432" MajorTopicYN="N">Metabolomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010420" MajorTopicYN="N">Pentanes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Fourier transform ion cyclotron resonance mass spectrometry</Keyword><Keyword MajorTopicYN="N">Populus × canescens</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>03</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>03</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24738572</ArticleId><ArticleId IdType="doi">10.1111/pce.12348</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Albert, Andreas" sort="Albert, Andreas" uniqKey="Albert A" first="Andreas" last="Albert">Andreas Albert</name><name sortKey="Barta, Csengele" sort="Barta, Csengele" uniqKey="Barta C" first="Csengele" last="Barta">Csengele Barta</name><name sortKey="Ghirardo, Andrea" sort="Ghirardo, Andrea" uniqKey="Ghirardo A" first="Andrea" last="Ghirardo">Andrea Ghirardo</name><name sortKey="Heller, Werner" sort="Heller, Werner" uniqKey="Heller W" first="Werner" last="Heller">Werner Heller</name><name sortKey="Kanawati, Basem" sort="Kanawati, Basem" uniqKey="Kanawati B" first="Basem" last="Kanawati">Basem Kanawati</name><name sortKey="Loreto, Francesco" sort="Loreto, Francesco" uniqKey="Loreto F" first="Francesco" last="Loreto">Francesco Loreto</name><name sortKey="Schmitt Kopplin, Philippe" sort="Schmitt Kopplin, Philippe" uniqKey="Schmitt Kopplin P" first="Philippe" last="Schmitt-Kopplin">Philippe Schmitt-Kopplin</name><name sortKey="Schnitzler, Jorg Peter" sort="Schnitzler, Jorg Peter" uniqKey="Schnitzler J" first="Jörg-Peter" last="Schnitzler">Jörg-Peter Schnitzler</name><name sortKey="Winkler, Jana Barbro" sort="Winkler, Jana Barbro" uniqKey="Winkler J" first="Jana Barbro" last="Winkler">Jana Barbro Winkler</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Kaling, Moritz" sort="Kaling, Moritz" uniqKey="Kaling M" first="Moritz" last="Kaling">Moritz Kaling</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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